Petuni is Punio's little sister in Paper Mario: The Thousand-Year Door. She needs rescuing by Punio and Mario, as she is locked up, with the majority of the other Punies, in a cage. She is later rescued by unlocking the cage with the red key. She then gives Punio a Dried Shroom, which had originally been an ordinary Mushroom. She had been saving to give to him as a present him but it shrivelled since her capture. Punio eats it but nearly chokes on it. He hides his choking by saying that it was delicious *Gag*.
After you save the Great Tree, Mario can play "Stump Petuni", a game in which you ask Petuni questions. If you ask her the question, "Mario likes which person the most?" She will answer correctly, "Princess Peach" and will give Mario a Mystic Egg. If Mario asks her any other question she will not know the answer.
Petuni participates in the 101 Punies that help Mario through the Great Tree, though she cannot always be seen in the group. Petuni is with the rest of the Punies when Mario leaves, and appears at the games end where she along with Punio gives Mario and Peach mushrooms.
| Punis |
|---|
| Punio | Petuni | Puni Elder | Puniper Pungry | Pungent | Punderton |