Kamek is one of Bowser's highest ranking henchmen in the Koopa Troop. He briefly makes an appearance at Bowser's side in the intro for Super Paper Mario, and plays a key role in Paper Mario: Sticker He can be randomly encountered in Paper Mario: Color Splash and appears as a mini-boss.

In Paper Mario: Sticker Star, he makes a larger appearance as a main antagonist. He makes his first appearance in World 1-4, when he battles you and you win, but then he takes the Sticker Comet Piece. He appears again in 2-2, where he is fighting you again, and turns your stickers into Flip Flops. After you win, you get the tablet piece. In World 3-3, he is seen splitting Wiggler into parts, so Mario and Kersti won't defeat Gooper Blooper. However, his plan fails in the end. He re-appears in World 4-3, where he broke the seal of the Book of Sealing and released all the Boos. After Mario and Kersti seal them all back by defeating them, Kamek comes out of hiding and releases the Boos again, and they turn into Big Boo, who Mario must defeat. His last appearance is in Bowser's Sky Castle, where he makes clones of himself and turns Mario's stickers into Flip Flops, then, after being defeated, he vanishes.

In Paper Mario: Color Splash, Kamek occasionally appears in random battles, and will modify Mario's cards. He can make them all Worn-Out, make them flip over, (the player doesn't know which card is which), randomize them, and even steal them. Once the battle has finished, the spell Kamek puts on the cards is reverted. His appearance within the story is in the Green Region. One Mario gets through the giant coin Kamek has used to block off the forest, He finds out Kamek has made Mondo Woods huge, and the Sacred Forest tiny. Once Mario and Huey get through both locations, they find Kamek guarding a Green Mini Paint Star and fight him. Kamek disables the Battle Spin, and must be fought without it. Once defeated, he tries to put a spell on Mario but fails. He surrenders, and grants Mario a fourth Card Slot (Presumably dying in the process).